izvor podataka: poirot

Glikozilacija plazmatskih proteina u razumijevanju dijabetesa tipa 1

Human plasma glycome in comprehension of type 1 diabetes

Glycosylation is a posttranslational modification that provides great proteomic diversity and often impact protein function. This process is regulated by a network of genes, many of which show pleiotropy with different diseases. Glycans present a great potential diagnostic tool as well as contribute to the understanding of the aetiology of diseases. The importance of glycosylation in type 1 diabetes mellitus (T1D) has not been addressed by now. T1D is a form of diabetes that results from the autoimmune destruction of the insulin-producing beta cells in the pancreas. Lack of care for these patients can be lethal, so any new knowledge about the development of this disease would represent an important contribution. Integrating glycomic changes with the subjects’ known genotypes could generate powerful and important results, potentially defining groups based on their glycome and genomic characteristics. This information may also be used to predict an unaffected individual’s risk of disease. This is why, in this study, we will combine expertise in genetic analysis and analyses of plasma glycome in order to search for the glycomic changes that are specific to T1D and for which we can correlate glycome and genome. To reach the goals of this study, we will analyze plasma glycomes of families with both diabetic siblings and unaffected siblings. Due to the age-related changes in glycosylation, parents will not be included in this study, so over 4500 samples will be analysed. All these samples have their genotyping performed. Glycomes will be analysed using MALDI-TOF-MS technology, which provides reliable analysis and presents the best available glycoanalytic tool. Thus, due to the undeniable role of glycans in pathological conditions, the well characterized population selected for this study and the use of highly modern and sensitive technology, this study will result in important new insights that will advance the comprehension and management of type 1 diabetes mellitus.

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Znanstveno-istraživački projekti





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HRK 966.850,00